Coding
Part:BBa_K3519000:Design
Designed by: Omkar Mohapatra Group: iGEM20_IISER-Tirupati_India (2020-10-07)
sulX
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 23
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 448
Illegal AgeI site found at 576 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is RFC 10 compatible. This part has not been codon optimised.
Source
Microbacterium sp. CJ77 sulX gene for sulfonamide monooxygenase SulX, complete CDS
References
Kim DW, Thawng CN, Lee K, Wellington EMH, Cha CJ. A novel sulfonamide resistance mechanism by two-component flavin-dependent monooxygenase system in sulfonamide-degrading actinobacteria. Environ Int. 2019 Jun;127:206-215. doi: 10.1016/j.envint.2019.03.046. Epub 2019 Mar 28. PMID: 30928844.